iHOPE Screening
Contact: Harumi Aono, Yushi Futamura E-mail: tech_CRAST
To discover bioactive small molecules ― bioprobes, we have constructed the in-house phenotypic evaluation (iHOPE) system where the biological activities, such as cytotoxicity, differentiation, morphology, of test samples against several cancer cell lines, bacteria, pathogenic fungi, and a malaria parasite, can be exhaustively evaluated.
1. Assay Overview
Here, we examine anti-cancer, anti-microbial, anti-malaria activity of a sample by using following cells and assay methods. All assays are carried out in 384-well format.
(1) Anti-cancer Activity
The human cervix epidermoid carcinoma cell line HeLa and the human promyelocytic leukemia cell line HL-60 are used in the assay. Anti-proliferative activity of a sample against these cancer cells is evaluated by using WST-8 assay.
(2) Anti-microbial Activity
The following microorganisms are used as test strains in the assay; Staphylococcus aureus 209, Escherichia coli HO141, Candida albicans JCM1542, Aspergillus fumigatus Af293, and Pyricularia oryzae kita-1. Antimicrobial activity tests are carried out by a standard microdilution method.
(3) Anti-malarial Activity
Plasmodium falciparum 3D7 is used as a test strain in the assay. Anti-malarial activity of a test sample is evaluated by using pLDH assay.
2. About Samples
Not only pure compounds, but also microbial/plant extracts can be tested. Please prepare the sufficient amount of sample for the assay by referring the following table. Note that samples should be dissolved in DMSO.
| Sample Type | Method | Required amount |
|---|---|---|
| Compound | 8 serial dilutions, n=3 Maximum conc: 30 μM |
20 mg/mL, 25 μL (or 0.5 mg powder) |
Broth extracts | Broth equivalent, n=1 | 20 μL |
| Fraction | 0.1, 1, 10 μg/mL, n=1 | 10 mg/mL, 20 μL |
3. Call for Application
- Assay schedule: January & July (2 times/year)
*Samples should be deposited to NPDepo half a month before assay starts - Result report: About 3 months after sample acceptance
4. About Sample Deposition
Please deposit your samples to NPDepo. Contact npdepo
for more information.
