iHOPE Screening

   Contact: Harumi Aono, Yushi Futamura  E-mail: tech_CRAST

 To discover bioactive small molecules ― bioprobes, we have constructed the in-house phenotypic evaluation (iHOPE) system where the biological activities, such as cytotoxicity, differentiation, morphology, of test samples against several cancer cell lines, bacteria, pathogenic fungi, and a malaria parasite, can be exhaustively evaluated.

1. Assay Overview

 Here, we examine anti-cancer, anti-microbial, anti-malaria activity of a sample by using following cells and assay methods. All assays are carried out in 384-well format.

(1) Anti-cancer Activity

 The human cervix epidermoid carcinoma cell line HeLa and the human promyelocytic leukemia cell line HL-60 are used in the assay. Anti-proliferative activity of a sample against these cancer cells is evaluated by using WST-8 assay.

(2) Anti-microbial Activity

 The following microorganisms are used as test strains in the assay; Staphylococcus aureus 209, Escherichia coli HO141, Candida albicans JCM1542, Aspergillus fumigatus Af293, and Pyricularia oryzae kita-1. Antimicrobial activity tests are carried out by a standard microdilution method.

(3) Anti-malarial Activity

 Plasmodium falciparum 3D7 is used as a test strain in the assay. Anti-malarial activity of a test sample is evaluated by using pLDH assay.

2. About Samples

 Not only pure compounds, but also microbial/plant extracts can be tested. Please prepare the sufficient amount of sample for the assay by referring the following table. Note that samples should be dissolved in DMSO.

Table. Assay method for each sample
Sample Type Method Required amount
Compound 8 serial dilutions, n=3
Maximum conc: 30 μM
20 mg/mL, 25 μL
(or 0.5 mg powder)
Broth extracts Broth equivalent, n=1 20 μL
Fraction 0.1, 1, 10 μg/mL, n=1 10 mg/mL, 20 μL

3. Call for Application

  • Assay schedule: January & July (2 times/year)
     *Samples should be deposited to NPDepo half a month before assay starts
  • Result report: About 3 months after sample acceptance

4. About Sample Deposition

 Please deposit your samples to NPDepo. Contact npdepo for more information.

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